The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle

Tjok Gde Oka Pemayun; Kadek Karang Agustina; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Putu Jodi Wiraguna Tangkas; I Nyoman Bagus Tri Aribawa

doi:10.5455/JRVS.20250913092012

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Original Article
Online Published: 13 Mar 2026

J Res Vet Sci. 2026; 6(1): 119-126

doi: 10.5455/JRVS.20250913092012

The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle

Tjok Gde Oka Pemayun, Kadek Karang Agustina, I Gusti Ngurah Bagus Trilaksana, Desak Nyoman Dewi Indira Laksmi, Putu Jodi Wiraguna Tangkas, I Nyoman Bagus Tri Aribawa.

Abstract
Aim: Repeat breeding syndrome (RBS) is a reproductive disorder in cattle characterized by failure to conceive after three or more inseminations with fertile semen in the absence of detectable abnormalities. Delayed ovulation is a major underlying cause. Conventional therapy using synthetic gonadotropins is constrained by cost, regulatory restrictions, and concerns about hormone residues in animal products. Therefore, this study aimed to evaluate the effectiveness of bovine pituitary extract (BPE), a natural source of gonadotropins derived from anterior pituitary glands, in managing RBS associated with delayed ovulation.
Methods: An experimental study with a Completely Randomized Design was conducted using Bali cattle diagnosed with RBS, divided into control (n = 16) and treatment (n = 16) groups. Estrus was monitored and follicle diameter measured ultrasonographically at estrus and prior to artificial insemination (AI). Twelve hours after estrus detection, the control group received 5 mL aqua bi-distillations, while the treatment group received 2 mL BPE diluted in 3 mL aqua bi-distillations. Follicle diameter data were analyzed using ANOVA and independent t-tests, and conception rates were compared using chi-square analysis (p < 0.05).
Results: Mean follicle diameters at estrus did not differ significantly between groups (p > 0.05). At AI, follicle size remained unchanged in controls but decreased significantly in the treatment group (p < 0.05), indicating ovulation induction.
Conclusion: BPE effectively induces ovulation in Bali cattle with RBS caused by delayed ovulation and represents a promising, biologically based alternative to synthetic hormonal therapy.

Key words: Cattle, infertility, ovulation, pituitary gland extracts, reproductive techniques

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Pubmed Style

Pemayun TGO, Agustina KK, Trilaksana IGNB, Laksmi DNDI, Tangkas PJW, Aribawa INBT. The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. J Res Vet Sci. 2026; 6(1): 119-126. doi:10.5455/JRVS.20250913092012

Web Style

Pemayun TGO, Agustina KK, Trilaksana IGNB, Laksmi DNDI, Tangkas PJW, Aribawa INBT. The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. https://www.wisdomgale.com/jrvs/?mno=283573 [Access: March 16, 2026]. doi:10.5455/JRVS.20250913092012

AMA (American Medical Association) Style

Pemayun TGO, Agustina KK, Trilaksana IGNB, Laksmi DNDI, Tangkas PJW, Aribawa INBT. The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. J Res Vet Sci. 2026; 6(1): 119-126. doi:10.5455/JRVS.20250913092012

Vancouver/ICMJE Style

Pemayun TGO, Agustina KK, Trilaksana IGNB, Laksmi DNDI, Tangkas PJW, Aribawa INBT. The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. J Res Vet Sci. (2026), [cited March 16, 2026]; 6(1): 119-126. doi:10.5455/JRVS.20250913092012

Harvard Style

Pemayun, T. G. O., Agustina, . K. K., Trilaksana, . I. G. N. B., Laksmi, . D. N. D. I., Tangkas, . P. J. W. & Aribawa, . I. N. B. T. (2026) The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. J Res Vet Sci, 6 (1), 119-126. doi:10.5455/JRVS.20250913092012

Turabian Style

Pemayun, Tjok Gde Oka, Kadek Karang Agustina, I Gusti Ngurah Bagus Trilaksana, Desak Nyoman Dewi Indira Laksmi, Putu Jodi Wiraguna Tangkas, and I Nyoman Bagus Tri Aribawa. 2026. The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. Journal of Research in Veterinary Sciences, 6 (1), 119-126. doi:10.5455/JRVS.20250913092012

Chicago Style

Pemayun, Tjok Gde Oka, Kadek Karang Agustina, I Gusti Ngurah Bagus Trilaksana, Desak Nyoman Dewi Indira Laksmi, Putu Jodi Wiraguna Tangkas, and I Nyoman Bagus Tri Aribawa. "The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle." Journal of Research in Veterinary Sciences 6 (2026), 119-126. doi:10.5455/JRVS.20250913092012

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Pemayun, T. G. O., Agustina, . K. K., Trilaksana, . I. G. N. B., Laksmi, . D. N. D. I., Tangkas, . P. J. W. & Aribawa, . I. N. B. T. (2026) The Efficacy of Bovine Pituitary Extract in the Management of Repeat Breeding Syndrome in Bali Cattle. Journal of Research in Veterinary Sciences, 6 (1), 119-126. doi:10.5455/JRVS.20250913092012

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