E-ISSN 3041-4296
 

Research Article

Online Publishing Date:
21 / 05 / 2024



Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12

Rupkatha Mukherjee, Debabrata Bera.


Abstract
Purified L-Asparaginase from Brevibacillus borstelensis ML12 was immobilized, and sequentially studied for biochemical characterization and thermodynamic feasibility. Immobilization has long been used as a way to enhance enzyme stability, facilitate recovery, and multiple re-uses in industries. It is preferable to utilize natural nontoxic carriers and crosslinking agents to immobilize enzymes. L-Asparaginase is an important industrial enzyme due to its significant role in the pharmaceutical and food sectors. In this study, immobilization of purified L-Asparaginase from a novel bacterium Brevibacillus borstelensis ML12 was performed using different carrier matrices and concentration of the suitable matrix was varied to obtain maximum enzyme production. Biochemical parameters like effect of pH, temperature, surfactants, organic reagents, bile salt were also studied. Arrhenius plot and thermal stability of immobilized enzyme were obtained from thermodynamic studies. ΔH, ΔG and ΔS values were also determined. Operational and storage stability studies revealed that the enzyme was able to retain 80% of its activity after 3 cycles and relative activity falls by 45% after 48 hours under refrigerated conditions and by 50% at normal room temperature. Vmax and KM values were calculated by using Lineweaver-Burk plot and the values came out to be 121.654 µmole ml-1 min-1 and 0.310 mM respectively.

Key words: L-Asparaginase, immobilization, kinetic parameters, enzyme stability


 
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Pubmed Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. 2024; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919


Web Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. https://www.wisdomgale.com/ejsrr/?mno=198199 [Access: May 24, 2024]. doi:10.5455/EJSRR.20240418110919


AMA (American Medical Association) Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. 2024; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919



Vancouver/ICMJE Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. (2024), [cited May 24, 2024]; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919



Harvard Style

Mukherjee, R., Bera, D. & (2024) Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919



Turabian Style

Mukherjee, Rupkatha, Debabrata Bera, and . 2024. Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. European Journal of Scientific Research and Reviews, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919



Chicago Style

Mukherjee, Rupkatha, Debabrata Bera, and . "Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12." European Journal of Scientific Research and Reviews 1 (2024), 69-79. doi:10.5455/EJSRR.20240418110919



MLA (The Modern Language Association) Style

Mukherjee, Rupkatha, Debabrata Bera, and . "Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12." European Journal of Scientific Research and Reviews 1.1 (2024), 69-79. Print. doi:10.5455/EJSRR.20240418110919



APA (American Psychological Association) Style

Mukherjee, R., Bera, D. & (2024) Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. European Journal of Scientific Research and Reviews, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919