E-ISSN 3041-4296
 
2024, Vol: 2, Issue: 1
2 / 1Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article
Online Published: 21 May 2024


Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12

Rupkatha Mukherjee, Debabrata Bera.


Abstract
Purified L-Asparaginase from Brevibacillus borstelensis ML12 was immobilized, and sequentially studied for biochemical characterization and thermodynamic feasibility. Immobilization has long been used as a way to enhance enzyme stability, facilitate recovery, and multiple re-uses in industries. It is preferable to utilize natural nontoxic carriers and crosslinking agents to immobilize enzymes. L-Asparaginase is an important industrial enzyme due to its significant role in the pharmaceutical and food sectors. In this study, immobilization of purified L-Asparaginase from a novel bacterium Brevibacillus borstelensis ML12 was performed using different carrier matrices and concentration of the suitable matrix was varied to obtain maximum enzyme production. Biochemical parameters like effect of pH, temperature, surfactants, organic reagents, bile salt were also studied. Arrhenius plot and thermal stability of immobilized enzyme were obtained from thermodynamic studies. ΔH, ΔG and ΔS values were also determined. Operational and storage stability studies revealed that the enzyme was able to retain 80% of its activity after 3 cycles and relative activity falls by 45% after 48 hours under refrigerated conditions and by 50% at normal room temperature. Vmax and KM values were calculated by using Lineweaver-Burk plot and the values came out to be 121.654 µmole ml-1 min-1 and 0.310 mM respectively.

Key words: L-Asparaginase, immobilization, kinetic parameters, enzyme stability


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Rupkatha Mukherjee
Articles by Debabrata Bera
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. 2024; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919

Web Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. https://www.wisdomgale.com/ejsrr/?mno=198199 [Access: October 11, 2024]. doi:10.5455/EJSRR.20240418110919

AMA (American Medical Association) Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. 2024; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919


Vancouver/ICMJE Style

Mukherjee R, Bera D, . Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev. (2024), [cited October 11, 2024]; 1(1): 69-79. doi:10.5455/EJSRR.20240418110919


Harvard Style

Mukherjee, R., Bera, D. & (2024) Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. Eu J Sci Res Rev, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919


Turabian Style

Mukherjee, Rupkatha, Debabrata Bera, and . 2024. Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. European Journal of Scientific Research and Reviews, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919


Chicago Style

Mukherjee, Rupkatha, Debabrata Bera, and . "Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12." European Journal of Scientific Research and Reviews 1 (2024), 69-79. doi:10.5455/EJSRR.20240418110919


MLA (The Modern Language Association) Style

Mukherjee, Rupkatha, Debabrata Bera, and . "Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12." European Journal of Scientific Research and Reviews 1.1 (2024), 69-79. Print. doi:10.5455/EJSRR.20240418110919


APA (American Psychological Association) Style

Mukherjee, R., Bera, D. & (2024) Immobilization and biochemical characterization of purified L-Asparaginase produced by Brevibacillus borstelensis ML12. European Journal of Scientific Research and Reviews, 1 (1), 69-79. doi:10.5455/EJSRR.20240418110919


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About European Journal of Scientific Research and Reviews

European Journal of Scientific Research and Reviews  is an international, peer-reviewed, open access journal, providing a platform for advances in ba ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

Your comments are very important to us

We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below.
 

Emails:

info@wisdomgale.com
editorinchief@ejmanager.com

Office Address:
WisdomGale Publishing
14 Rue de Grand-Bigard,
1082 Brussels
Belgium
 
We will respond within 48 to 72 hours.
 
Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.