E-ISSN 3041-4180
 

Original Article
Online Published: 08 Jan 2025
 


Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria

Abraham Isah, Abdulrazak Baba Ibrahim, Rebeccah Wusa Ndana, Yoila David Malann, Florence Onyeje Abraham, Victoria Shiru Ndana, Rose Suniso Maxwel Gidado, Lateef Ariyo Adeniran, Joy Onwude.


Abstract
The commercial release of genetically engineered (GE) crops has improved agricultural productivity in many countries. Currently, only three crops have been commercially available in Nigeria. However, there are allegations on the possible illegal existence of many or few more GE crops that have not been commercially approved by the government due to what observers regard as weak regulatory system. The release and commercialization of GE crops in Nigeria must contend with the possibility of having GE crops already in circulation. This study sought to probe the possible existence of the un-commercialised GE crops in major markets of Northern Nigeria, by targeting herbicide tolerance- phosphinothricin acetyltransferase PAT and insect resistance- Pesticidal crystal proteins (Cry) traits. Soybean (Glycine max), cowpea (Vigna unguiculata) and maize (Zea mays) seeds were collected, and lateral flow strip method used to screen for possible transgenic events in those crops. No transgenic event was found in any of the samples tested using flow-strip analysis. PCR analysis also revealed no positive transgenic event. This is the first report on a scientific investigation to detect Cry and Pat in grains in the region. The approach used in this study serves to provide a baseline data on the status of GE events in Nigeria.

Key words: GE crops, Bacillus thuringiensis, Cry, PAT, transgenic events, Biosafety


 
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How to Cite this Article
Pubmed Style

Isah A, Ibrahim AB, Ndana RW, Malann YD, Abraham FO, Ndana VS, Gidado RSM, Adeniran LA, Onwude J. Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. J Res Agric Food Sci. 2025; 2(3): 54-64. doi:10.5455/JRAFS.20240924023605


Web Style

Isah A, Ibrahim AB, Ndana RW, Malann YD, Abraham FO, Ndana VS, Gidado RSM, Adeniran LA, Onwude J. Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. https://www.wisdomgale.com/jrafs/?mno=221761 [Access: January 28, 2025]. doi:10.5455/JRAFS.20240924023605


AMA (American Medical Association) Style

Isah A, Ibrahim AB, Ndana RW, Malann YD, Abraham FO, Ndana VS, Gidado RSM, Adeniran LA, Onwude J. Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. J Res Agric Food Sci. 2025; 2(3): 54-64. doi:10.5455/JRAFS.20240924023605



Vancouver/ICMJE Style

Isah A, Ibrahim AB, Ndana RW, Malann YD, Abraham FO, Ndana VS, Gidado RSM, Adeniran LA, Onwude J. Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. J Res Agric Food Sci. (2025), [cited January 28, 2025]; 2(3): 54-64. doi:10.5455/JRAFS.20240924023605



Harvard Style

Isah, A., Ibrahim, . A. B., Ndana, . R. W., Malann, . Y. D., Abraham, . F. O., Ndana, . V. S., Gidado, . R. S. M., Adeniran, . L. A. & Onwude, . J. (2025) Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. J Res Agric Food Sci, 2 (3), 54-64. doi:10.5455/JRAFS.20240924023605



Turabian Style

Isah, Abraham, Abdulrazak Baba Ibrahim, Rebeccah Wusa Ndana, Yoila David Malann, Florence Onyeje Abraham, Victoria Shiru Ndana, Rose Suniso Maxwel Gidado, Lateef Ariyo Adeniran, and Joy Onwude. 2025. Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. Journal of Research in Agriculture and Food Sciences, 2 (3), 54-64. doi:10.5455/JRAFS.20240924023605



Chicago Style

Isah, Abraham, Abdulrazak Baba Ibrahim, Rebeccah Wusa Ndana, Yoila David Malann, Florence Onyeje Abraham, Victoria Shiru Ndana, Rose Suniso Maxwel Gidado, Lateef Ariyo Adeniran, and Joy Onwude. "Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria." Journal of Research in Agriculture and Food Sciences 2 (2025), 54-64. doi:10.5455/JRAFS.20240924023605



MLA (The Modern Language Association) Style

Isah, Abraham, Abdulrazak Baba Ibrahim, Rebeccah Wusa Ndana, Yoila David Malann, Florence Onyeje Abraham, Victoria Shiru Ndana, Rose Suniso Maxwel Gidado, Lateef Ariyo Adeniran, and Joy Onwude. "Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria." Journal of Research in Agriculture and Food Sciences 2.3 (2025), 54-64. Print. doi:10.5455/JRAFS.20240924023605



APA (American Psychological Association) Style

Isah, A., Ibrahim, . A. B., Ndana, . R. W., Malann, . Y. D., Abraham, . F. O., Ndana, . V. S., Gidado, . R. S. M., Adeniran, . L. A. & Onwude, . J. (2025) Lateral Flowstrip and PCR detection approach for the identification of unapproved transgenic crops in northern Nigeria. Journal of Research in Agriculture and Food Sciences, 2 (3), 54-64. doi:10.5455/JRAFS.20240924023605