Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.

Lallawm Sangi; William Carrie; Lalhruaitluanga Hauhnar; Esther Lalnunmawii

doi:10.5455/JBRR.20260305024329

J Biol Res Rev. 2026; 3(3): 112-127

doi: 10.5455/JBRR.20260305024329

Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.

Lallawm Sangi, William Carrie, Lalhruaitluanga Hauhnar, Esther Lalnunmawii.

Abstract
Aim: The study aimed at studying, identifying, and evaluating the bioactive properties of endophytic bacteria isolated from the medicinal plant Dillenia pentagyna Roxb.
Methods: An agar well diffusion assay was employed for antimicrobial screening. 16S rRNA gene sequencing was done to identify selected bacteria. Antioxidant assays using DPPH and ABTS radical scavenging assays were performed. Cytotoxicity was tested using the MTT assay, and detection of secondary metabolites was done using HPTLC.
Results: Among the 96 isolates, one endophytic bacterium, BPSL8, identified as Bacillus pumilus, had the most significant activity. The crude methanol extract of BPSL8 exhibited antibacterial activity against Micrococcus luteus, Bacillus subtilis, and Klebsiella pneumoniae. BPSL8 has a potent antioxidant capacity of DPPH and ABTS free radicals with an IC₅₀ of 6.29 and 4.25 µg/mL, respectively. BPSL8 was found to have cytotoxicity against HT29 (IC50 55.2 µg/mL) and HeLa (IC50 51.9 µg/mL). HPTLC analysis done for three fractions (DCM, EA, and MeOH) of BPSL8 detected the presence of phytochemicals.
Conclusion: This study demonstrates the potential of the identified endophytic bacterium B. pumilus as a source of as a potent antibacterial, antioxidant, and anticancer agent with a key role in providing insights into their impact on the host as well as their applications in the field of pharmaceuticals.

Key words: Antimicrobial, Bacillus, Bioactive compounds, Endophytes, HPTLC

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How to Cite this Article

Pubmed Style

Sangi L, Carrie W, Hauhnar L, Lalnunmawii E. Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. J Biol Res Rev. 2026; 3(3): 112-127. doi:10.5455/JBRR.20260305024329

Web Style

Sangi L, Carrie W, Hauhnar L, Lalnunmawii E. Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. https://www.wisdomgale.com/jbrr/?mno=312805 [Access: July 27, 2026]. doi:10.5455/JBRR.20260305024329

AMA (American Medical Association) Style

Sangi L, Carrie W, Hauhnar L, Lalnunmawii E. Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. J Biol Res Rev. 2026; 3(3): 112-127. doi:10.5455/JBRR.20260305024329

Vancouver/ICMJE Style

Sangi L, Carrie W, Hauhnar L, Lalnunmawii E. Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. J Biol Res Rev. (2026), [cited July 27, 2026]; 3(3): 112-127. doi:10.5455/JBRR.20260305024329

Harvard Style

Sangi, L., Carrie, . W., Hauhnar, . L. & Lalnunmawii, . E. (2026) Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. J Biol Res Rev, 3 (3), 112-127. doi:10.5455/JBRR.20260305024329

Turabian Style

Sangi, Lallawm, William Carrie, Lalhruaitluanga Hauhnar, and Esther Lalnunmawii. 2026. Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. Journal of Biological Research and Reviews, 3 (3), 112-127. doi:10.5455/JBRR.20260305024329

Chicago Style

Sangi, Lallawm, William Carrie, Lalhruaitluanga Hauhnar, and Esther Lalnunmawii. "Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.." Journal of Biological Research and Reviews 3 (2026), 112-127. doi:10.5455/JBRR.20260305024329

MLA (The Modern Language Association) Style

Sangi, Lallawm, William Carrie, Lalhruaitluanga Hauhnar, and Esther Lalnunmawii. "Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.." Journal of Biological Research and Reviews 3.3 (2026), 112-127. Print. doi:10.5455/JBRR.20260305024329

APA (American Psychological Association) Style

Sangi, L., Carrie, . W., Hauhnar, . L. & Lalnunmawii, . E. (2026) Bioactive potential of endophytic Bacillus pumilus BPSL8 isolated from the medicinal plant Dillenia pentagyna Roxb.. Journal of Biological Research and Reviews, 3 (3), 112-127. doi:10.5455/JBRR.20260305024329