Research Article

Online Publishing Date:
03 / 06 / 2024



Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays

Rutuja Sanjay Desai, Sameer Jahangeer Nadaf, Ashwini Ramakrishana Gawade, Ashwin Bhanudas Kuchekar.


Abstract
Aim: The study aims to evaluate the anti-angiogenic and cytotoxic properties of Carissa carandas Linn., a medicinal plant traditionally used in India for various ailments.
Method: The plant's leaf extracts were prepared using methanol and n-hexane solvents and subjected to phytochemical analysis, revealing the presence of compounds like tannins, triterpenoids, and flavonoids. The antioxidant potential of the extracts was assessed, with the fruit extract showing remarkable scavenging activity against free radicals. The anti-angiogenic activity was examined using the Chorioallantoic Membrane (CAM) assay. Cytotoxicity was determined through the Brine Shrimp Lethality Assay (BSL.
Results: The Chorioallantoic Membrane (CAM) assay demonstrated significant inhibition of blood vessel formation by the plant extracts. Cytotoxicity assays indicated that both methanol and n-hexane extracts contain potent bioactive constituents, albeit less potent than the standard potassium dichromate.
Conclusion: The findings suggest that Carissa carandas Linn. possesses significant anti-angiogenic and cytotoxic properties, supporting its traditional use in medicine and highlighting its potential as a source of novel anticancer compounds. The study underscores the importance of scientific validation of medicinal plants' therapeutic properties and contributes to the growing interest in natural products for cancer treatment.

Key words: Anti-angiogenic activity, Cytotoxicity, Carissa Carandas Linn., Chorioallantoic Membrane Assay, Brine Shrimp Lethality Assay.


 
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How to Cite this Article
Pubmed Style

Desai RS, SJN, Gawade AR, Kuchekar AB. Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. J Cancer Res Rev. 2024; 1(1): 30-39. doi:10.5455/JCRR.20240512042153


Web Style

Desai RS, SJN, Gawade AR, Kuchekar AB. Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. https://www.wisdomgale.com/jcrr/?mno=201370 [Access: June 08, 2024]. doi:10.5455/JCRR.20240512042153


AMA (American Medical Association) Style

Desai RS, SJN, Gawade AR, Kuchekar AB. Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. J Cancer Res Rev. 2024; 1(1): 30-39. doi:10.5455/JCRR.20240512042153



Vancouver/ICMJE Style

Desai RS, SJN, Gawade AR, Kuchekar AB. Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. J Cancer Res Rev. (2024), [cited June 08, 2024]; 1(1): 30-39. doi:10.5455/JCRR.20240512042153



Harvard Style

Desai, R. S., , . S. J. N., Gawade, . A. R. & Kuchekar, . A. B. (2024) Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. J Cancer Res Rev, 1 (1), 30-39. doi:10.5455/JCRR.20240512042153



Turabian Style

Desai, Rutuja Sanjay, Sameer Jahangeer Nadaf, Ashwini Ramakrishana Gawade, and Ashwin Bhanudas Kuchekar. 2024. Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. Journal of Cancer Research and Reviews, 1 (1), 30-39. doi:10.5455/JCRR.20240512042153



Chicago Style

Desai, Rutuja Sanjay, Sameer Jahangeer Nadaf, Ashwini Ramakrishana Gawade, and Ashwin Bhanudas Kuchekar. "Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays." Journal of Cancer Research and Reviews 1 (2024), 30-39. doi:10.5455/JCRR.20240512042153



MLA (The Modern Language Association) Style

Desai, Rutuja Sanjay, Sameer Jahangeer Nadaf, Ashwini Ramakrishana Gawade, and Ashwin Bhanudas Kuchekar. "Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays." Journal of Cancer Research and Reviews 1.1 (2024), 30-39. Print. doi:10.5455/JCRR.20240512042153



APA (American Psychological Association) Style

Desai, R. S., , . S. J. N., Gawade, . A. R. & Kuchekar, . A. B. (2024) Investigation of Anti-Angiogenic and Cytotoxic Properties of Carissa Carandas Linn. Extracts Using In Vivo and In Vitro Assays. Journal of Cancer Research and Reviews, 1 (1), 30-39. doi:10.5455/JCRR.20240512042153