Abstract
Background and Aim: Crude sewage water poses a risk to the environment as well as to human health. The aim of this study is to identify the microbes responsible for the sewage water pollution in various areas of Taraba State.
Method: The total of nine samples of sewage water was screened using nested PCR with broad-range primer pairs targeting the DNA of the presumptive organisms from the sewage samples of three drainage sites.
Results: The sewage water site's greatest and the lowest physicochemical temperature parameter is 310C and 270C respectively. The bacterial counts in sewage ranged from 3.3105cfu/mL to 0.5105cfu/mL while the fungal counts varied between 1.8×105sfu/mL to 0.4×105sfu/mL. Micrococcus, Piscinibacter, Bacillus, Neisseria, Pseudomonas, Staphylococcus, Streptomyces, Vagococcus, Lysinibacillus, Streptococcus, Aspergillus, Rhizopus, Cladosporium, Penicillium and Yeast are the genera of bacterial and fungal that was frequently isolated during the study. Based on DNA sequencing analysis and phylogenic analysis, the organisms DNA sequences had 88.86% – 99.86% nucleotide identification to the sequences from the isolated organisms which include; Lysinibacillus sphaericus, Streptococcus infantarius, Vagococcus fluvialis, Baccilus tropicus and Staphylococcus aureus.
Conclusion: Depending on how actively people participate and how long they spend in the sewage water, microbial infections may be transmitted to residents. Hence, it is recommended that sewage systems be carefully planned and located away from residential areas. In order to preserve a healthy environment, proper sewage disposal must be followed.