Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.

Farzana  Afroz; hasnaif Mohammad Shifat; mst. Deloara  Begum; mir Rowshan  Akter; md. Mominul  Islam

doi:10.5455/EJMID.20241024053102

Eu J Microbiol Infect Dis. 2024; 1(4): 139-146

doi: 10.5455/EJMID.20241024053102

Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.

Farzana Afroz, Hasnaif Mohammad Shifat, Mst. Deloara Begum, Mir Rowshan Akter, Md. Mominul Islam.

Cited By:4

Abstract
Aims: Extended-spectrum beta-lactam (ESBL) antibiotics resistance is one of the top antimicrobial threats globally. Recent evidence suggests the emergence of ESBL-positive Escherichia coli from the animal origin foods. However, information on the animal origin ESBL-positive E. coli is very limited in Bangladesh. The present study investigated the frequency and characteristics of ESBL-positive E. coli in milk samples from cows with mastitis in northern Bangladesh.
Methods: The phenotype of ESBL-positive E. coli was determined using ESBL-CHROM agar media and the antibiotic sensitivity was measured by disk diffusion method. PCR was used to detect the E. coli and ESBL-genes of the isolates.
Results: This study detected 60 E. coli isolates from 400 milk samples. Among the isolates, ESBL-positive E. coli was detected in 14 (23.33%) isolates according to the results of phenotypic confirmatory methods. Our findings further demonstrated that the most common ESBL gene was blaCTX-M (92.86%), subsequently 71.43% of the isolates had blaTEM. Most of the isolates bearing blaCTX-M gene also harbored with blaTEM gene (78.57%). Interestingly, blaSHV gene was absent in all isolated E. coli strains. Antibiogram revealed 100% of ESBL-positive isolates showed multidrug-resistant (MDR) in nature and exhibited complete resistance to ampicillin. Additionally, 92.86% were resistant to cefuroxime, cefaclor, and cefotaxime, while 85.72% showed resistance to cefpodoxime, amoxicillin-clavulanic acid, and aztreonam. Notably, all isolates were 100% sensitive to carbapenems.
Conclusion: This study provides noble evidence on the animal borne ESBL producing E. coli and raises need for vigilant antibiotic stewardship and robust infection control measures.

Key words: Escherichia coli, mastitis, antibiotic resistance, multidrug resistance

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Farzana Afroz Articles by hasnaif Mohammad Shifat Articles by mst. Deloara Begum Articles by mir Rowshan Akter Articles by md. Mominul Islam
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Afroz F, Shifat HM, Begum MD, Akter MR, Islam MM. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis. 2024; 1(4): 139-146. doi:10.5455/EJMID.20241024053102

Web Style

Afroz F, Shifat HM, Begum MD, Akter MR, Islam MM. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. https://www.wisdomgale.com/ejmid/?mno=225891 [Access: June 07, 2026]. doi:10.5455/EJMID.20241024053102

AMA (American Medical Association) Style

Afroz F, Shifat HM, Begum MD, Akter MR, Islam MM. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis. 2024; 1(4): 139-146. doi:10.5455/EJMID.20241024053102

Vancouver/ICMJE Style

Afroz F, Shifat HM, Begum MD, Akter MR, Islam MM. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis. (2024), [cited June 07, 2026]; 1(4): 139-146. doi:10.5455/EJMID.20241024053102

Harvard Style

Afroz, F., Shifat, . H. M., Begum, . M. D., Akter, . M. R. & Islam, . M. M. (2024) Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis, 1 (4), 139-146. doi:10.5455/EJMID.20241024053102

Turabian Style

Afroz, Farzana, Hasnaif Mohammad Shifat, Mst. Deloara Begum, Mir Rowshan Akter, and Md. Mominul Islam. 2024. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. European Journal of Microbiology and Infectious Diseases, 1 (4), 139-146. doi:10.5455/EJMID.20241024053102

Chicago Style

Afroz, Farzana, Hasnaif Mohammad Shifat, Mst. Deloara Begum, Mir Rowshan Akter, and Md. Mominul Islam. "Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.." European Journal of Microbiology and Infectious Diseases 1 (2024), 139-146. doi:10.5455/EJMID.20241024053102

MLA (The Modern Language Association) Style

Afroz, Farzana, Hasnaif Mohammad Shifat, Mst. Deloara Begum, Mir Rowshan Akter, and Md. Mominul Islam. "Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.." European Journal of Microbiology and Infectious Diseases 1.4 (2024), 139-146. Print. doi:10.5455/EJMID.20241024053102

APA (American Psychological Association) Style

Afroz, F., Shifat, . H. M., Begum, . M. D., Akter, . M. R. & Islam, . M. M. (2024) Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. European Journal of Microbiology and Infectious Diseases, 1 (4), 139-146. doi:10.5455/EJMID.20241024053102